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KMID : 0545120020120061002
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 6 p.1002 ~ p.1005
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Overproduction of Bacillus macerans Cyclodextrin Glucanotransferase in E. coli by Coexpression of GroEL/ES Chaperone
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KWON, MI-JUNG
PARK, SO-LIM/KIM, SUNG-KOO/NAM, SOO-WAN
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Abstract
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The effects of GroEL/ES chaperone on the production of soluble from of B. macerans cyclodextrin glucanotransferase (CGTase) in recombinant E. coli were investigated. The cgt gene and groEL/ES genes are under the control of T7 promoter and Pzt-1 promoter, respectively. The optimal concentrations of inducers, IPTG and tetracycline, were found to be 1.0 mM and 10ng/ml, respectively. When tetracycline and IPTG were added at the early exponential phase (2h) and early exponential phase (3h) of growth, respectively, about increase of soluble CGTase protein were obtained. An SDS-PAGE analysis revealed that about 37.2% of total CGTase protein was in the soluble fraction when GroEL/ES chaperone was overexpressed.
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